書名 DNA replication : methods and protocols / edited by Sonya Vengrova, Jacob Dalgaard
出版項 New York : Humana Press, c2015
版本項 2nd ed
book jacket
館藏地 索書號 條碼 處理狀態
 陽明大學書庫區  QU475 D629 2015    30020100800137  在架上
稽核項 x, 295 p. : ill. (some col.) ; 27 cm
叢書名 Methods in molecular biology, 1064-3745 ; 1300
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 1300. 1064-3745
Springer protocols (Series) 1949-2448
內容 A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- High-Resolution Analysis of Mammalian DNA Replication Units -- Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast -- Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection -- Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo -- Detection and Sequencing of Okazaki Fragments In S. cerevisiae -- ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin -- Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors -- Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism -- Single-Molecule Observation of Prokaryotic DNA Replication -- Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
附註 Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories
主題 DNA replication -- Research -- Methodology
DNA replication -- Laboratory manuals
DNA Replication
其他作者 Vengrova, Sonya
Dalgaard, Jacob Z
國際標準書號 9781493925957 (hbk.) : EUR94.99
9781493925964 (ebk.)